Implemented kill switches: 3
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
| Name |
Number |
Comment |
| T4 Holin |
BBa_K112805 |
Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer |
| T4 endolysin |
BBa_K112806 |
The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer |
Antagonists
| Name |
Number |
Comment |
| Antiholin |
BBa_K112807 |
Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria |
Topology
Parts
| Name |
Number |
Comment |
| AHL receptor lysis |
BBa_K317039 |
complete AHL-mediated lysis system |
Kill Switch 2
Class
# Format:
# Write how you would classify them.
Toxins
| Name |
Number |
Comment |
| T4 Holin |
BBa_K112805 |
Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer |
| T4 endolysin |
BBa_K112806 |
The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer |
Antagonists
| Name |
Number |
Comment |
| Antiholin |
BBa_K112807 |
Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria |
Topology
Parts
| Name |
Number |
Comment |
| AHL receptor lysis |
BBa_K317040 |
complete IPTG-mediated lysis system |
Kill Switch 3
Class
# Format:
# Write how you would classify them.
Toxins
| Name |
Number |
Comment |
| T4 Holin |
BBa_K112805 |
Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer |
| T4 endolysin |
BBa_K112806 |
The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer |
Antagonists
| Name |
Number |
Comment |
| Antiholin |
BBa_K112807 |
Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria |
Topology
Parts
| Name |
Number |
Comment |
| lux pR |
BBa_R0062 |
This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer (3-oxo-hexanoyl-HSL) |
| luxR repressor/activator |
BBa_C0062 |
In complex with HSL and LuxR binds to the Lux promoter |
| Enterobacteria phage T4 Lysis Device |
BBa_K112808 |
This is the lysis device using three genes from enterobacteria phage T4: lysozyme and holin and antiholin |